Section I. Chemical Biology -- Determinants of Nitric Oxide Chemistry: Impact of Cell Signaling Processes -- Nitric oxide redox biochemistry in lipid environments -- Mechanisms and biological consequences of peroxynitrite-dependent protein oxidation and nitration -- Systems Approaches to Unraveling Nitric Oxide Response Networks in Prokaryotes -- Section II. Principles of Biology -- A. Nitric Oxide Synthases -- Uncoupling of endothelial NO synthase (eNOS) in cardiovascular disease and its pharmacological reversal -- Tetrahydrobiopterin: An essential cofactor for Nitric Oxide Synthases and Amino Acid Hydroxylases -- Regulation of the expression of inducible nitric oxide synthase -- Molecular Regulation of Inducible Nitric Oxide Synthase -- B. Guanylate Cyclase and Cyclic GMP --Soluble Guanylate Cyclase: Allosteric Activation and Redox Regulation -- C. Nitric Oxide Signaling -- Untargeted Discovery of NO-modified Proteins -- Fatty Acid Transduction of Nitric Oxide Signaling: Cyclooxygenases, Lipoxygenases and Nitro-Fatty Acids -- Nitric Oxide Signaling in Vascular Cells is Regulated through CD47 by Thrombospondin-1 -- The Regulation of Cell Energetics and Mitochondrial Signaling by Nitric Oxide -- D. Regulation of Nitric Oxide Production -- Nitric oxide-asymmetric dimethylarginine system in endothelial cell senescence -- E. Nitric Oxide and Remodeling -- The Role of Nitric Oxide in Apoptosis and Autophagy: Biochemical and Computational Studies -- F. Nitrite and Nitrate -- NO formation from inorganic nitrate and nitrite -- Mechanisms of nitrite reduction in ischemia in the cardiovascular system: therapeutic potential -- Nitrite Therapy for Ischemic Syndromes -- Nitrite and heme globins: Reaction mechanisms and physiological targets -- Section III. Principles of Pathobiology -- A. Nitric Oxide and Cardiovascular Function -- Nitric Oxide in Vascular Damage and Regeneration -- Free radicals as atherosclerotic risk-The relation of NO- -- The role of oxidative stress in endothelial dysfunction and vascular inflammation -- B. Pathophysiological Mechanisms of Nitric Oxide -- Nitric oxide, oxidative stress, immune response and critical care -- Reactive Metabolites of Oxygen and Nitrogen in Liver Ischemia and Reperfusion Injury -- Nitric oxide in airway inflammation -- Novel therapeutic applications of nitric oxide (NO) in the inhibition of tumor malignancy and reversal of resistance.